科研相關服務
蛋白質表達服務
其它科研服務
試劑盒原料開發
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多克隆抗體定制服務
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多克隆抗體能夠識別多個抗原表位，并且由于制備周期短，成本低，應用廣泛等優點已成為科研人員進 行免疫學研究不可或缺的重要工具。戴安生物憑借5000+多抗項目研發和生產經驗，可以提供兔、大鼠、小鼠、豚鼠、 羊、雞等多個宿主在內的多抗制備。并且已成功定制過以下多種屬蛋白質抗體：人、小鼠、大鼠、斑馬魚、銀鯽、草魚、 黃顙魚、鱖魚、海參、海膽、線蟲、、四膜蟲、細菌、病毒、擬南芥、藍藻、棉花、玉米、水稻、馬鈴薯、油菜、小麥等，實戰經驗非常豐富，提供多個物種，多種抗原，多個應用平臺的多克隆抗體定制，能夠最大程度保證抗體定制的成功率。
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服務步驟 |
具體內容 | 周期 | 交付 | 報價 |
| 抗原分析及設計 | 分析蛋白免疫原性，親水性，同源性等選擇合適的特異性序列 | 1個工作日 | 分析報告 | 免費分析 |
| 抗原制備 | 蛋白質路線，密碼子優化合成基因，可溶性表達蛋白質 | 3-4周 |
100ug蛋白質小樣 或 1mg多肽小樣 |
參考蛋白表達或多臺合成 |
| 對特定的蛋白結合位點序列設計合成多肽，并與KLH偶聯 | ||||
| 動物免疫 | 每只宿主進行5次免疫，包括初次免疫(加入完全弗氏佐劑)和4次加強免疫(加入不完全弗氏佐劑) | 65天（可調整短周期） | 兔抗血清60ml（其他宿主另計），200 μL兔免疫前陰性血清（有需要請備注） | 詢價 |
| 效價檢測 | 公司檢測ELISA效價，將免疫原包被進行ELISA檢測 | 1個工作日 | 項目服務報告 | 詢價 |
| 抗體純化 | 抗原親和純化或者Protein A / G純化。 | 7個工作日 | 5-10mg親和純化的抗體 | 詢價 |
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| 案 例 展 示 |
瓊擴法檢測抗體效價 免疫熒光檢測多抗特異性
| 已 發 表 文 獻（部分） | |||||
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[1] Xiaobo Yu,et al. Fertilized egg cells secrete endopeptidases to avoid polytubey. Nature. 31 March.2021 [2] Zhaojing Lu ,et al. Deletion of the transmembrane protein Prom1b in zebrafish disrupts outer-segment morphogenesis and causes photoreceptor degeneration .JBC. July 30, 2019 [3] Meng-Jie Zhao,et al. MiR-15b and miR-322 inhibit SETD3 expression to repress muscle cell differentiation [J]. Cell Death & Disease. 22 February 2019 [4] Miao Li1,2,3*, Tao Huang1,2,3*, Meng-Jing Li1,2,3*, et al. The histone modification reader ZCWPW1 is requiredfor meiosis prophase I in male but not in female mice [J]. Science Advances 14 Aug 2019: Vol. 5, no. 8, eaax1101 [5] Functional characterization of IL-10 and its receptor subunits in a perciform fish, the mandarin fish, Siniperca chuatsi [J]. Developmental & Comparative Immunology. Volume 97, August 2019, Pages 64-75 [6] Yao Wang1 ,et al. Mitochondria-localised ZNFX1 functions as a dsRNA sensor to initiate antiviral responses through MAVS [J].Nature Cell Biology 2019 Nov;21(11):1346-1356. [7] Linlin Shi 1, Weimin Wang 1 and Yanhe Li 1,4,*et al. Molecular Characterization and Functional Study of Insulin-Like Androgenic Gland Hormone Gene in the Red Swamp Crayfish, Procambarus clarkii[J]. MDPI Genes 2019 [8] Jing Hou, Shan Nan Chen, Zhen Gan, Nan Li, Lin Huang, Hui Jun Huo, Yue Cong Yang, Yishan Lu, Zhan Yin and Pin Nie In Primitive Zebrafish, MHC Class II Expression Is Regulated by IFN-γ, IRF1, and Two Forms of CIITA J Immunol 2020 May 1;204(9):2401-2415 [9]JunyanTao, Chenglian Bai, Yuanhong Chen, Huanhuan Zhou, Yahui Liu, Qingyu Shi, WenhaoPan, Haojia Dong, Luyi Li, Hui Xu, Robyn Tanguay, Changjiang Huang, QiaoxiangDong Environmental relevant concentrations of benzophenone-3 induced developmental neurotoxicity in zebrafish[J].Science of The Total Environment 2020 [10]Qiannan Zhang1 , Yanghong Xu1, Jishuai Huang1, Kai Zhang2, Haijun Xiao3 8 ,XiaojianQin4, Linlin Zhu5, Yingguo Zhu1,#, and Jun Hu1* Therice pentatricopeptide repeat protein PPR756 is involved in pollen developmentby affecting multiple RNA editing in mitochondria. Front Plant Sci 2020 Jun 12;11:749 [11] Xinfeng Li et al. Clp protease and antisense RNA jointly regulate the global regulator CarD to mediate mycobacterial starvation response Elife. 2022 [12] Xinfeng Li et al. Ccdc38 is required for sperm flagellum biogenesis and male fertility in mouse Biorxiv January 11, 2022. |
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